RIO 100

Innovation

New Innovative RIO Raman sampling optics allow you to develop the next generation of Raman applications by collecting high-quality Raman spectra from more complex solids, turbid liquids, and clear liquids.

Representative Sampling - "Collimated Laser with Backscatter Collection"

Probe optic-based alternative to the PhAT Probe and Transmission Raman as it uses diffuse scattering and photon migration within the sample to enhance the Raman signal and provide representative sampling while at the same time reducing the unwanted Raman signal from surface coatings.

Improved Spectral Precision - "Real-Time Raman Calibration"

Unwanted wavenumber shifts observed in Raman spectra from the laser or spectrograph due to thermal drifting or instability can identified and corrected for by using the superimposed Raman calibration band from the reference calibration optic.

Raman Signal Enhancement - "Probe Optic Light Shield"

The gold-plated inner surface of the probe optic shield enhances the Raman signal from a turbid liquid sample by extending the laser pathlength due to the high (>90% ) reflectivity of the inner gold plating redirecting the scattered laser and Raman signal back into the sample.

Products

Zero X Plus

Zero X Plus

The Zero X Plus microscope objective offers "Collimated Laser with Backscatter Collection" to provide Representative Sampling and "Real-Time Raman Calibration" to provide Improved Spectral Precision enabling improved qualitative and quantitative analysis of complex solid samples using a research Raman microscope.

RIO 50 Plus

RIO 50 Plus

This probe optic uses diffuse scattering and photon migration within the bulk of the sample to enhance the Raman signal and generate representative sampling. This technique relates closely to the approach used by a PhAT Probe and Transmission Raman instrument.

RIO 100

RIO 100

With the gold-plated RIO 100 Probe Optic Light Shield and integrated retroreflector the Raman signal is enhanced by extending the laser pathlength using diffuse scattering and photon migration from the particles within the turbid liquid sample.

Zero X Plus

Quantitative Measurements and Improved Spectral Precision

The Zero X Plus microscope objective incorporates "Collimated Laser with Backscatter Collection" to provide "Representative Sampling" and "Real-Time Raman Calibration" to provide Improved Spectral Precision allowing for improved qualitative and quantitative analysis of complex solid samples using a traditional research Raman microscope.

  • Closely related to the PhAT Probe and Transmission Raman techniques as both rely on diffuse scattering and photon migration within the sample to enhance the Raman signal and provide representative sampling.
  • Unwanted Raman signals from surface coatings are significantly reduced making it ideal for coated tablets and capsules.
  • Extended Wavenumber Range: The Zero X Plus optic enables the collection of representative spectra over the complete Raman spectral range (5 to 3425 cm-1)
  • "Real-Time Raman Calibration" can correct for unwanted wavenumber shifts observed in Raman spectra from the laser or spectrograph due to thermal drifting or instability.

Reduced Unwanted Signal from Surface Coatings

The Raman spectra below collected from a generic Excedrin tablet using a research Raman microscope with a focusing 20X objective. The surface coating generates a strong unwanted background signal

Figure 1

The Raman spectra below was collected from the same generic tablet using "Collimated Laser with Backscatter Collection" on the same Raman microscope. The collimated laser is transmitted through the surface coating and the Raman spectra from the bulk of the sample dominates the combined Raman spectra as per Transmission Raman.

Figure 2

Representative Sampling

The Zero X Plus on a Raman microscope will enable representative sampling from the "Collimated Laser with Backscatter Collection" design over the full Raman spectral range (5 to 3425 cm-1) and not just the limited wavenumber ranges of the PhAT probe and Transmission Raman. Below RIO 50 Plus (Blue Spectra) and PhAT Probe (Red Spectra)

Figure 3

Real-Time Calibration

The 321.0 wavenumber band shown in the plot below from the CaF2 standard is outside of the 400-1800 wavenumber chemical fingerprint region and the 5-200 wavenumber Terahertz region reducing the probability for overlapping of analytically important Raman bands from the sample with the calibration band. Flat spectral response outside of CaF2 calibration band avoids unwanted Raman bands from sapphire windows or lenses.

Figure 4

Features

  • Representative Sampling: Simply switch from traditional focused point measurements to Zero X Plus large spot measurements to enable representative sampling using "Collimated Laser with Backscatter Collection" generating reproducible measurements and representative sampling.
  • Extended Wavenumber Range: The Zero X Plus optic enables the collection of representative spectra over the complete Raman spectral range (5 to 3425 cm-1)
  • Long Working Distance: The Zero X Plus optic enables the non-contact collection of Raman spectra from samples inside deep wellplates.
  • Large Spot Size: The Zero X Plus optic enables signal averaging over SERS surfaces.
  • Real-Time Calibration: An integrated CaF2 optic generates a reference Raman band at 321.0 wavenumbers superimposed onto each collected Raman spectra.
  • Improved Spectral Precision: Will allow for spectral separation of each component compared to the traditional spatial separation of individual components.
  • Upgrade Option: The Zero X Plus microscope objective can be used on most Raman microscopes.

Applications

Qualitative analysis by spectral separation of individual components and chemometric modeling for concentration predictions.

  • Coated tablets
  • Uncoated Tablets
  • Capsules
  • Powders
  • Blends
  • Microplastics
  • SERS substrates

References

RAMAN IMMERSION PROBE SYSTEMS AND METHODS
U.S. Patent – 10,976,259 B2
REAL-TIME RAMAN CALIBRATION
US Patent Application Serial No. 17/386,723

Collimated Laser with Backscatter Collection

The technique uses diffuse scattering and photon migration within the bulk of the sample to enhance the Raman signal and generate representative Raman spectra enabling the Quantitative Analysis of Solids and Powders. This technique relates closely to the approach used by a PhAT Probe and Transmission Raman instrument.

Features

  • The RIO 50 Plus optic can enable the collection of representative spectra over the complete Raman spectral range (5 to 3425 cm-1)
  • The RIO 50 Plus optic has a long working distance and depth of field making it ideal for both Non-contact or Immersible applications.
  • The RIO 50 Plus optic is a retrofit option for use with most existing Raman instruments.

RIO 50 Plus Probe Optic

RIO 50 Plus - Immersion or Non-Contact Optic

Applications

Qualitative and Quantitative measurements on:

  • Complex Mixtures
  • Powders
  • Blends
  • Granules
  • Slurries

Probe Comparison

RIO 50 plus (Blue Spectra) versus PhAT Probe (Red Spectra)

RIO 50 Plus vs PhAT Probe

Real-Time Raman Calibration

Unwanted wavenumber shifts observed in Raman spectra from the laser or spectrograph due to thermal drifting or instability can be corrected for by using the Raman reference band at 321.0 cm-1 superimposed onto each collected Raman spectra.

Features

  • Improved spectral precision will improve the sensitivity and robustness of chemical models and calibration transfer between different probes and instruments.
  • The 321.0 cm-1 reference Raman band lies between the 5-200 cm-1 Terahertz region and the 400-1800 cm-1 chemical fingerprint region.
  • The overall flat spectral response avoids unwanted Raman bands generated by sapphire windows or lenses

RIO 50 Plus Probe Optic

RIO 50 Plus - Immersion or Non-Contact Optic

Applications

Qualitative and Quantitative measurements on:

  • Complex Mixtures
  • Powders
  • Blends
  • Granules
  • Slurries

Raman Reference Band

The 321.0 w avenumber Raman band in the plot below is from the integrated CaF2 optic

Raman Reference Band

Probe Optic Light Shield

The RIO 100 is an immersion optic that collects enhanced Raman spectra using diffuse scattering from particles and photon migration within turbid liquids. The gold-plated RIO 100 Probe Optic Light Shield with retroreflector enhances the Raman signal by extending the laser pathlength by diffuse scattering from particles within the turbid liquid sample.

Features

The inner and outer slots on the surface of the probe optic light shield are offset to allow for liquid flow and to provide:

  • Improved laser safety
  • Reduced interference from unwanted outside lighting
  • Reduced laser induced fluorescence from glass reactors

RIO 100 Probe Optic

RIO 100 with Retroreflector

Applications

Potential applications are:

  • Nucleation processes
  • Crystallization processes
  • Upstream fermentation
  • Downstream fermentation
  • Nanoparticles
  • Micro-plastics

Gold-Plated Probe Optic Light Shield

Gold Plated Shield RIO 100 with Gold Plated Optic Light Shield

RIO Probe Optics Product Guide

The RIO 50 Plus, RIO 100, RIO 100 Probe Optic Shield, RIO 150L Plus, and RIO 150S Plus Raman sampling optics are designed to collect high-quality Raman spectra from more complex solids, turbid liquids, and clear liquids.

Sample Type Probe Type Optical Design Benefits Applications
Solids RIO 50 Plus Collimated Laser with Backscatter Collection Representative Sampling With Real-Time Calibration Powders, Blends, Granules, Tablets
Liquids with Solids RIO 100 Collimated Laser with Backscatter Collection and Retroreflector Representative Sampling Colloids, Suspensions, Slurries
Liquids with Solids RIO 100 Probe Optic Light Shield Collimated Laser with Backscatter Collection and Retroreflector Raman Signal Enhancement Nucleation, Crystallization
Liquids with Solids RIO 150S Plus f/2 Short Focus Fast collection With Real-Time Calibration Suspensions, Colloids
Liquids RIO 150L Plus f/2 Short Focus Fast collection With Real-Time Calibration Clear Liquids

HORC Raman Patent Portfolio

Granted Patent - U.S. 10,746,596 B2
Probe Optic Light Shields

Granted Patent - US 10,976,259 B2
Raman Immersion Probe Systems and Methods

Granted Patent - US 11,086,123 B2
Improved Raman Immersion Probe Optics

Patent Application - Serial No. 63/058,714
Real Time Raman Calibration

Patent Application - Serial No. 63/122,261
Solids Analysis using Raman Spectroscopy

Biography

Harry Owen a recognized expert in the field of Raman spectroscopy is now director of his own company focusing on pursuing his passion for invention and innovation by creating and patenting new Raman products and applications for existing and new markets.

He was Born in Manchester, UK, is married to Susan has 3 sons and is currently residing in Michigan, USA.

Education

MSc. Solid State Physics, University of London
Grad. Inst. Physics, John Dalton College of Technology

Experience

As Vice President and Director of Business Development at Kaiser Optical Systems he founded a successful new business utilizing Raman spectroscopy to develop a range of innovative spectroscopic components and instruments.

Awards

"Recognized as a pioneer in the Development of Scientific Instrumentation: Raman Spectroscopy" at the Fourteenth James L Waters Annual Symposium at the 2003 Pittsburgh Conference.

James L. Waters Award

Top Left: M. Bonner Denton, Charles W. Gardner, Bruce Chase, Harry Owen.
Bottom Left: James L Waters, Fran Adar.

The Williams-Wright Award from the Coblentz Society at the 2006 Pittsburgh Conference for the development of novel holographic optical components designed to enable the development of a new generation of compact and robust Raman instruments.

Williams Wright Award

Left: Don Pivonka, Harry Owen, Bob Messerschmidt

Bruno Lenain

Left: The late Bruno Lenain, a true Raman pioneer and business partner.

Granted Patents

A track record for invention across 4 different scientific fields is supported by the award of 13 US patents from 1978 to 2017.

Raman Applications

US Patent 10,139,329
Particle Size Determination using Raman Spectroscopy

US Patent 6,867,858
Raman spectroscopy crystallization analysis method

Raman Instrumentation

US Patent 5,377,004
Remote optical measurement probe.

US Patent 5,011,284
Detection system for Raman scattering employing holographic diffraction

US Patent 8,675,190
Large-collection-area Raman probe with reduced background fluorescence

US Patent 7,148,963
Large-collection-area optical probe

Holographic Optical Elements

US Patent 5,559,597
Spectrograph with multiplexing of different wavelength regions onto a single opto-electric detector array

US Patent 5,530,565
Holographic transmission bandpass filter.

Photochromic Glasses

US Patent 4,349,634
Method of preparing photochromic glass.

US Patent 4,290,813
Photochromic boro-silicate glass.

US Patent 4,102,693
Photochromic boro-silicate glass.

Contact

Primary

All countries not covered by exclusive partners.

Full name
Harry Owen
Company
HORC
Email address
harry@horc.us
Telephone
+1 248-933-6935
Address
Michigan, USA

Exclusive Partner

for Japan

Full name
Dr. Hiroshi Hisada
Company
Tekanalysis Inc.
Email address
hisada@tekanalysis.jp
Telephone
+81 90 4037 2065
Address
Osaka, Japan

Exclusive Partner

for all of Ireland

Full name
Donnchadha Quilty
Company
Particular Sciences Ltd.
Email address
donq@particular.ie
Address
Dublin, Ireland

Exclusive Partner

for Korea

Full name
Kon-Pyo, Hong
Company
Sunil-Ina Instrument Co., Ltd.
Email address
sunilina@sunilina.com
Address
5, Dongwon-ro 21Beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do, 13547 Korea

Exclusive Partner

for England, Scotland, and Wales

Full name
John Andrews
Company
Clairet Scientific Ltd.
Email address
info@clairet.co.uk
Address
Northampton, NN3 6AP, UK